Cloned (Comment) | Organism |
---|---|
expression in escherichia coli and Pichia pastoris, His-Patch-thioredoxin-TPH, 6His-TPH or as a fusion protein with maltose-binding protein | Homo sapiens |
General Stability | Organism |
---|---|
tryptophan and glycerol stabilize | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(6R)-5,6,7,8-tetrahydrobiopterin | - |
Homo sapiens | |
dithiothreitol | - |
Homo sapiens | |
Fe2+ | - |
Homo sapiens | |
L-tryptophan | substrate inhibition above 0.1 mM | Homo sapiens | |
additional information | no substrate inhibition by (6R)-L-erytho-5,6,7,8-tetrahydrobiopterin | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
L-tryptophan | TPH as fusion protein with maltose-binding protein expressed in Escherichia coli | Homo sapiens | |
0.0185 | - |
L-tryptophan | TPH expressed in Pichia pastoris | Homo sapiens | |
0.027 | - |
(6R)-L-erytho-5,6,7,8-tetrahydrobiopterin | TPH as fusion protein with maltose-binding protein expressed in Escherichia coli | Homo sapiens | |
0.039 | - |
(6R)-L-erytho-5,6,7,8-tetrahydrobiopterin | TPH expressed in Pichia pastoris | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0214 | - |
TPH expressed in Pichia pastoris | Homo sapiens |
0.296 | - |
TPH as fusion protein with maltose-binding protein expressed in Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + (6R)-L-erytho-5,6,7,8-tetrahydrobiopterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPH | - |
Homo sapiens |